28 — 7 (45) 2022 — Barnoev Akhtam Istamovich, Khasanova Dilnoza Ahrorovna, CHANGES IN MORPHOMETRIC PARAMETERS OF THE SMALL INTESTINE IN EXPERIMENTAL LUNG FIBROSIS

CHANGES IN MORPHOMETRIC PARAMETERS OF THE SMALL INTESTINE IN EXPERIMENTAL LUNG FIBROSIS

Barnoev Akhtam Istamovich, Bukhara State Medical Institute named after Abu Ali Ibn Sino

Khasanova Dilnoza Ahrorovna, Bukhara State Medical Institute named after Abu Ali Ibn Sino

Resume

The aim is to study the histological features of the small intestine in white rats with experimental pulmonary fibrosis.

Materials and methods: To solve the tasks, 131 laboratory white male rats, weighing 120-150 grams, served in vivarium conditions. All the animals were divided into two groups. In the first group there were 36 intact rats. The second group consisted of 95 mature males, the effect of experimental lung fibrosis on the tissue structures of the small intestine was studied. For general morphological studies, pieces of tissue from 3 sections (WPC, skinny and iliac) of the small intestine were fixed in a neutral 10% solution of formalin, Carnois fluid. After the appropriate wiring, the material was poured into paraffin. Further, sections 4-6 microns thick with the strictest orientation after dewaxing were stained with hematoxylin-eosin for morphological and morphometric studies.

Results. Morphological characteristics of tissue structures of the small intestine in experimental pulmonary fibrosis, inflammatory and destructive changes in the form of intercellular edema and infiltration of intestinal tissue structures were observed throughout the small intestine. After 30-60 days with experimental pulmonary fibrosis, destructive and atrophic changes worsen, the height of the villi decreases and, accordingly, the number of epithelial cells in the villi.

In the late stages of the study, atrophic processes progress in all layers of the intestinal wall, especially in the mucous membrane. The villi acquire a bizarre shape, especially in the jejunum. However, in some places there is a development of compensatory and restorative processes. In these areas, swelling and infiltration decrease, villi and crypts acquire a normal shape, and their morphometric parameters approach the control indicators.Conclusions. In experimental pulmonary fibrosis, certain structural changes occur, characterized by the development of inflammatory and destructive changes in the tissue structures of the small intestine. The beginning of stabilization of processes was noted up to 60 days. The nature of changes in the tissue structures of all layers of the wall of the small intestine is especially pronounced in the mucous membrane, duodenum and jejunum

Key words: experimental fibrosis, small intestine, lymphoid follicles

First page

135

Last page

139

For citation: Barnoev Akhtam Istamovich, Khasanova Dilnoza Ahrorovna, CHANGES IN MORPHOMETRIC PARAMETERS OF THE SMALL INTESTINE IN EXPERIMENTAL LUNG FIBROSIS //New Day in Medicine 7(45)2022 135-139 https://l.clck.bar/e1694

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