77-1 (33) 2021 — Sabirmatov A.A, Tursumetov A. A., — POSSIBILITIES OF METHYLENE BLUE IN PHOTODYNAMIC INACTIVATION OF BACTERIA IN VITRO EXPERIMENT

POSSIBILITIES OF METHYLENE BLUE IN PHOTODYNAMIC INACTIVATION OF BACTERIA IN VITRO EXPERIMENT

Sabirmatov A.A. -Tashkent Pediatric Medical Institute., Tashkent State Dental Institute khasanjafarov@gmail.com

Tursumetov A. A. -Tashkent Pediatric Medical Institute., Tashkent State Dental Institute khasanjafarov@gmail.com

Mukhamedov I.M. -Tashkent Pediatric Medical Institute., Tashkent State Dental Institute khasanjafarov@gmail.com

Jafarov Kh.M. -Tashkent Pediatric Medical Institute., Tashkent State Dental Institute khasanjafarov@gmail.com

 Iskhakov Sh. Sh. -Tashkent Pediatric Medical Institute., Tashkent State Dental Institute khasanjafarov@gmail.com

Resume

The paper presents the results of bench microbiological studies on the study of the antibacterial properties of red spectrum LED radiation with a wavelength of 630 ± 20 nm, the photosensitizer methylene blue (MB) in various concentrations. The objects of exposure were bacteria sown from hernial water in strangulated hernia and abdominal exudate in peritonitis (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli). It was found that LED radiation and MB, separately, do not possess bactericidal properties. Photodynamic effect (PDT) (LED radiation in combination with 0.05% MB solution) has a pronounced bactericidal effect. The obtained results are discussed from the point of view of the possibility of using PDT for the prevention of postoperative proinflammatory complications.

Key words: methylene blue, infection, photodynamic effect

First page

404

Last page

411

For citation: Sabirmatov A.A, Tursumetov A. A., Mukhamedov I.M., Jafarov Kh.M., Iskhakov Sh. Sh., Possibilities of methylene blue in photodynamic inactivation of bacteria in vitro experiment//New Day in Medicine 1(33)2021 404-411 https://cutt.ly/wcfsz7o

LIST OF REFERENCES

1. Gusarov V.G., Nesterova E.E., Lashenkova N.N. et al. Changes in antibiotic resistance of nosocomial microflora: results of the implementation of antimicrobial therapy control strategy in a multidisciplinary hospital. // Epidemiology and infectious diseases. 2015.Vol. 20.No. 5.P. 11-18.
2. Mashkovsky M. D. Medicines. M .: RIA “New Wave”: Publisher Umerenkov, 2008.1206s.
3. Shmigol T.A., Sobyanin K.A., Prusak- Glotov M.V. et al. // Application of antimicrobial photodynamic therapy based on MC 540 to a model of wound infection.
4. Stranadko E.F., Geynits A.V., Volgin V.N.// Clinical   and  socio-economic assessment of photodynamic therapy of cancer  and  non-tumor  disea8ses  //  Laser medicine. -2015. -№2. -Vol. 19. -S.37-41.
5. Stranadko E.F., Ryabov T.I. Photodynamic therapy is a new medical technology for the treatment   of   cancer   and   non-neoplastic diseases // Russian Biotherapeutic Journal. – 2016. -Т.15.-№1.-С.104-105.
6. Strakhovskaya M.G.,  Belenikina  N.S., Nikitina   V.V.  et   al. A  promising photosensitizer for antimicrobial photodynamic therapy // Clinical practice. – 2013.-№1.-С.25-30.
7. Fakayode  OJ,  Tsolekile  N,  Songca  SP, Oluwafemi     OS.      Applications    of functionalized nanomaterials  in photodynamic therapy. Biophys. 2018; 2. doi: 10.1007/s12551-017-0383-2.
8. Rundle P. Photodynamic Therapy for Eye Cancer. Biomedicines. 2017; 5 (4): 69. Hamblin MR. Antimicrobial photodynamic inactivation: a bright new technique to kill resistant microbes. Curr Opin Microbiol. 2016; 33: 67–73.