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  • 62 -7 (69) 2024 - Sayfutdinova Z.A., Akhmedova D.B. - RATIONALE FOR THE USE OF A NEW MIXTURE OF AMINO ACIDS FOR LIVER DAMAGE BASED ON PATHOPHYSIOLOGY

62 -7 (69) 2024 - Sayfutdinova Z.A., Akhmedova D.B. - RATIONALE FOR THE USE OF A NEW MIXTURE OF AMINO ACIDS FOR LIVER DAMAGE BASED ON PATHOPHYSIOLOGY

RATIONALE FOR THE USE OF A NEW MIXTURE OF AMINO ACIDS FOR LIVER DAMAGE BASED ON PATHOPHYSIOLOGY

Sayfutdinova Z.A. - Tashkent Medical Academy

Akhmedova D.B. - Tashkent Medical Academy

Abdukhakimova M.A. - Tashkent Medical Academy

Safarova G.A. - Tashkent Medical Academy

Meliboeva Kh.Sh. - Tashkent Medical Academy

Resume

Purpose of the study: Experimental study of the pathophysiological basis for the use of new mixtures of amino acids for the treatment of liver disease. Materials and methods: To achieve the goal, a model of toxic hepatitis was reproduced using heliotrine poisoning. Acute exposure to heliotrine was caused by subcutaneous administration of a sublethal dose of heliotrine to rats, calculated as 40 mg per 100 g of body weight. Toxic hepatitis is caused by the administration of heliotrine at a dose of 25 mg/100 g. Indicators of protein balance, biological materials (including ALT, AST, bilirubin, alpha-amylase) were studied by biochemical analysis using the HUMAN test system (Germany) on a BA88A biochemical analyzer (Mindray, China). Determination of protein fractions was carried out using the turbidimetric method. Research results. During the induction of experimental toxic hepatitis by administration of heliotrine, HIF-1 levels were observed to average 0.101667 ± 0.0022 ng/L. In blood plasma, the average levels of HIF-1 were 0.2136±0.0066 ng/l. These results can be explained by the effect of heliotrine on the liver, especially on hepatocytes, where mitochondria are starved of oxygen. Thus, HIF-1 acts as an early biomarker of tissue hypoxia, and its elevation in experimental ischemic animals may promote angiogenesis by promoting vascular proliferation necessary for oxygenation. In addition, during replication of experimental toxic hepatitis by heliotrine administration, mean ALT levels were 25.93 ± 2.91 U/L and AST levels were 22.23 ± 1.95 U/L. The de Riets coefficient was determined to be 1.17±0.16. The level of direct bilirubin was 3.90±0.44 mmol/l, and indirect bilirubin - 8.10±0.8 mmol/l. The level of total bilirubin was 12.01±1.16 mmol/l. The odds ratio (OR) was 0.93219976 with a 95% confidence interval (CI) of 0.88765239. The Wilcoxon test yielded a χ2 value of 0.9633286. The Mann-Whitney test (U-statistic) was 0.87219981 with a significance level of p<0.05. The data obtained indicate a direct correlation between protein balance indicators and oxygen deficiency caused by heliotrine. Conclusions: The developed amino acid mixture showed a higher effectiveness on the development and course of experimental toxic hepatitis compared to traditional methods of treatment, such as Infizol, which was confirmed by scientific research.

Key words: heliotrin intoxication; biomarkers of inflammation; amino acid mixture; Infezol; experimental animals

First page

354

Last page

359

For citation:Sayfutdinova Z.A., Akhmedova D.B., Abdukhakimova M.A., Safarova G.A., Meliboeva Kh.Sh. - RATIONALE FOR THE USE OF A NEW MIXTURE OF AMINO ACIDS FOR LIVER DAMAGE BASED ON PATHOPHYSIOLOGY//New Day in Medicine 7(69)2024 354-359 https://newdayworldmedicine.com/en/new_day_medicine/7-69-2024

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