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  • 45 -8 (70) 2024 - Kim T.A., Mavlyanova Z.F. - IMMUNOREACTIVE INSULIN AND GLUCOSE CONTENT IN BLOOD IN RATS WITH ALLOXAN DIABETES AGAINST LEAD INTOXICATION

45 -8 (70) 2024 - Kim T.A., Mavlyanova Z.F. - IMMUNOREACTIVE INSULIN AND GLUCOSE CONTENT IN BLOOD IN RATS WITH ALLOXAN DIABETES AGAINST LEAD INTOXICATION

IMMUNOREACTIVE INSULIN AND GLUCOSE CONTENT IN BLOOD IN RATS WITH ALLOXAN DIABETES AGAINST LEAD INTOXICATION

Kim T.A. - Kazakh National Medical University named after. S.D. Asfendiyarova, Almaty, Kazakhstan

Mavlyanova Z.F. - Samarkand State Medical University Uzbekistan

Khudoykulova F.V. - Samarkand State Medical University Uzbekistan

Resume

Aim: identification of the effect of subtoxic doses of lead acetate on the endocrine function of the pancreas. Materials and methods: The object of the study was 80 male outbred white rats weighing 250-280 g, divided into the following groups: Group 1 - 33 rats that received 1% lead acetate solution orally for 30 days - the "lead" group; Group 2 - 18 rats with alloxan diabetes against the background of lead exposure - the "lead + alloxan" group; Group 3 - 47 intact rats that received 0.9% NaCl solution orally for 30 days - the "intact" group (n = 47); Group 4 - alloxan diabetes in 32 intact rats - the "intact + alloxan" group (control). Fasting blood glucose and ketone body levels, immunoreactive insulin were studied in all animals. To study the processes of lipid peroxidation and the state of the antioxidant system, a homogenate was prepared from liver cells and the activity of superoxide dismutase was studied using the method of V.P. Verbolovich, and the activity of catalase using the method of M.A. Korolyuk. The activity of oxidative metabolism in hepatocyte membranes was determined by the content of diene conjugates and malonic dialdehyde using the method of E.I. Lvovskaya, I.A. Volegorodsky, and S.E. Shemyakov. The concentration of lead in the blood of rats was determined on the 30th day of inoculation using atomic emission spectrometry with inductively coupled plasma. Results: Alloxan diabetes in rats pre-treated with lead acetate for 30 days at a dose of 15 mg/kg body weight occurred less frequently compared to the animals of the control group, and was characterized by a milder course according to immunoreactive insulin and hyperglycemia indices. At the same time, there were 3 times more rats in the experimental group with glucose levels from 6.1 to 14.0 mmol/l on the third day after injection of alloxan, and 2.3 times more on the 14th day than in the control group. There were 1.8 times fewer rats with hyperglycemia over 14.1 mmol/l on the third day in the experimental group, and 2.7 times fewer on the 14th day than in the animals of the control group. On the third day after the injection of alloxan, activation of free-radical oxidation processes was observed in the liver of rats in the experimental and control groups: in the experimental group, the content of diene conjugates and malonic dialdehyde increased by 22% and 12%, respectively, while in the control, the level of diene conjugates increased by 39%, and malonic dialdehyde by 31% compared with the corresponding initial values. Also, in rats with alloxan diabetes against the background of lead exposure, higher activity of superoxide dismutase and slightly reduced activity of catalase were noted compared with animals with experimental alloxan diabetes without lead intoxication. Conclusions: the revealed milder course of alloxan diabetes in animals against the background of exposure to subtoxic doses of lead is pathogenesis associated with an increase in the activity of superoxide dismutase immediately at the time of administration of alloxan, and on the third day of the course of experimental alloxan diabetes.

Key words: rats, experiment, alloxan diabetes, immunoreactive insulin, glucose, intoxication, lead, pancreas

First page

244

Last page

251

For citation:Kim T.A., Mavlyanova Z.F., Khudoykulova F.V. - IMMUNOREACTIVE INSULIN AND GLUCOSE CONTENT IN BLOOD IN RATS WITH ALLOXAN DIABETES AGAINST LEAD INTOXICATION//New Day in Medicine 8(70)2024 244-251 https://newdayworldmedicine.com/en/new_day_medicine/8-70-2024

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