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  • 15 -10 (72) 2024 - Sayfutdinova Z.A., Islamova Z.S. - MECHANISMS CAUSING ISCHEMIC LIVER DAMAGE AND THEIR CORRECTION WITH A NEW AMINO ACID MIXTURE

15 -10 (72) 2024 - Sayfutdinova Z.A., Islamova Z.S. - MECHANISMS CAUSING ISCHEMIC LIVER DAMAGE AND THEIR CORRECTION WITH A NEW AMINO ACID MIXTURE

MECHANISMS CAUSING ISCHEMIC LIVER DAMAGE AND THEIR CORRECTION WITH A NEW AMINO ACID MIXTURE

Sayfutdinova Z.A.- Tashkent Pediatric Medical Institute

Islamova Z.S.- Tashkent Pediatric Medical Institute

Mirzamudova N.A.- Tashkent Pediatric Medical Institute

Xamidova U.E.- Tashkent Pediatric Medical Institute

Nurillayeva G.S.- Tashkent Pediatric Medical Institute

Resume

During the reproduction of the experimental toxic hepatitis by the introduction of heliotrin, it was found that the content of HIF-1 was on average 0.101667 ± 0.0022 ng / l. In blood plasma, the mean HIF-1 values were 0.2136 ± 0.0066 ng / L. After treatment, in group I, HIF-1α values were 0.317 ± 0.022 (p <0.01), in group II — 0.404 ± 0.031 (p≤0.02), in group III — 0.365 ± 0.026 (p≤0.001), in group IV group — 0.421 ± 0.028 (p≤0.001). During the reproduction of experimental toxic hepatitis by the introduction of heliotrin, it was found that the ALT content was on average 25.93 ± 2.91 U / L, and the AST content was at the level of 22.23 ± 1.95 U / L. The de Rits were at 1.17 ± 0.16. Direct bilirubin was at the level of 3.90 ± 0.44 mmol / L, indirect bilirubin — 8.10 ± 0.8 mmol / L. The total bilirubin was 12.01 ± 1.16 mmol / L. Moreover, OR (odds ratio) was 0.93219976. The 95% CI (confidence interval) was 0.88765239. χ 2 = 0.9633286 (Wilconson test). Mann-Winney test (U test) was 0.87219981 at p <0.05. Conclusions: The developed amino acid mixture is superior to traditional methods of treatment (Infezol) in terms of the effectiveness of influence on the development and course of experimental toxic hepatitis, which is proved by the study.

Key words: toxic hepatitis; amino acid mixtures; heliotrin intoxication; ischemia; hypoxia; Infezol.

First page

96

Last page

102

For citation:Sayfutdinova Z.A., Islamova Z.S., Mirzamudova N.A., Xamidova U.E., Nurillayeva G.S. - MECHANISMS CAUSING ISCHEMIC LIVER DAMAGE AND THEIR CORRECTION WITH A NEW AMINO ACID MIXTURE//New Day in Medicine 10(72)2024 96-102 https://newdayworldmedicine.com/en/new_day_medicine/10-72-2024

List of References

  1. Catal T., Tunali S., Bolkent S. An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-GalactosamineInduced Hepatotoxicity in Rats / T. Catal, S. Tunali, S. Bolkent //Eur J Biol. 2017;76(1):14-19.
  2. Chamuleau R. A. F. M. End-stage liver failure: flling the treatment gap at the intensive care unit /R. A. F. M. Chamuleau, R. Hoekstra //Journal of Artifcial Organs. 2019.
  3. Chen EY, Fujinaga M, Giaccia AJ. Hypoxic microenvironment within an embryo induces apoptosis and is essential for proper morphological development. //Teratology. 2019;60:215–225.
  4. Cramer T., Yamanishi Y., Clausen B.E., et al. HIF-1α is essential for myeloid cell-mediated inflammation. //Cell. 2013;112:645–657.
  5. Date T, Mochizuki S, Belanger AJ, et al. Expression of constitutively stable hybrid hypoxia-inducible factor-1α protects cultured rat cardiomyocytes against simulated ischemia-reperfusion injury. //Am J Physiol. 2015;288:314-320.
  6. Dong S. Mechanisms of CCl 4 -induced liver fibrosis with combined transcriptomic and proteomic analysis / S. Dong, Q. Chen, Y. Song //The Journal of Toxicological Sciences. 2016;4:561-572.
  7. Haytham EL Sayed Ali EL Sayed, Lotfy EL Sayed Morsy, Tamer Mosad Abo Emara Effect of Carbon Tetrachloride (CCL4) on Liver in Adult Albino Rats: Histological study / Haytham EL Sayed Ali EL Sayed, Lotfy EL Sayed Morsy, Tamer Mosad Abo Emara //The Egyptian Journal of Hospital Medicine. 2019;76(6):4254-4261.
  8. Forbes S. J. Liver Regeneration - Mechanisms and Models to Clinical Application / S. J. Forbes, Ph. N. Newsome //Nature Reviews Gastroenterology Hepatology. 2016;13(85):473-485.
  9. Fox S.B., Braganca J, Turley H, et al. CITED4 inhibits hypoxia-activated transcription in Cancer Cells, and its cytoplasmic location in breast cancer is associated with elevated expression of tumor cell hypoxia-inducible factor 1α //Cancer Res. 2014;64:6075-6081.
  10. Genbacev O, Zhou Y, Ludlow JW, Fisher SJ. Regulation of human placental development by oxygen tension. //Science. 2017;277:1669-1672.
  11. Grant T., McMillin M., Frampton G. Direct Comparison of the Thioacetamide and Azoxymethane Models of Type A Hеpatic Encephalopathy in mice / Grant T., McMillin M., Frampton G. //The Journal of Liver Research. 2018;3:171-185.
  12. Giannini E. G., Testa R., Savarino V. Liver enzyme alteration: a guide for clinicians //Canadian Medical Association Journal (CMAJ). 2015:172:367-379.
  13. Gilgenkrantz H. Understanding Liver RegenerationFrom Mechanisms to Regenerative Medicine /H. Gilgenkrantz, A.C. de l’Hortet //The American Journal of Pathology. 2018;188(6):1316-1327.
  14. Greenbaum L.E. Clinical translation of liver regeneration therapies: a conceptual road map / L. E.Greenbaum, C. Ukomadu, J. S.Tchorz //Biochemical Pharmacology. – 2020.
  15. Hepatic NF-kB-inducing kinase (NIK) suppresses mouse liver regeneration in acute and chronic liver diseases / Yi Xiong, A. Torsoni, Wu Feihua [et al.] //eLife. 2018;7:1-18.
  16. Hewitson K.S, Schofield C.J. The HIF pathway as a therapeutic target. //Drug Discov Today. 2014;9:704-711.
  17. Ho T.K., Rajkumar V, Ponticos M, et al. Increased endogenous angiogenic response and hypoxia-inducible factor-1α in human critical limb ischemia. //J Vasc Surg. 2016;43:125-133.
  18. Hua, Fu. CCL4 promotes the cell proliferation, invasion and migration of endometrial carcinoma by targeting the VEGF-A signal pathway / Fu Hua, Ye Tian //Int J Clin Exp Pathol. 2017:10(11):11288-11299.
  19. Maes M. Experimental models of hepatotoxicity related to acute liver failure / Michaël Maes, Mathieu Vinken, Hartmut Jaeschke //Toxicology and Applied Pharmacology. 2016;290:86-97.
  20. L.P. de Carvalho Batista Éboli, A.A. Salzedas Netto, R.A. de Azevedo Evaluating the Best Time to Intervene Acute Liver Failure in Rat Models Induced by D-Galactosamine / L. P. de Carvalho Batista Éboli, A. A. Salzedas Netto, R. A. de Azevedo //Acta Cirúrgica Brasileira. 2016:131(12):783-792.

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