111 -10 (72) 2024 - Matrizayeva G.Dzh., Ikhtiyarova G.A. - MODERN APPROACH TO DIAGNOSIS OF MISTARRIAGE

MODERN APPROACH TO DIAGNOSIS OF MISTARRIAGE

Matrizayeva G.Dzh. - Urgench branch of Tashkent Medical Academy (Republic of Uzbekistan)

Ikhtiyarova G.A. - Bukhara State Medical Institute (Republic of Uzbekistan)

Resume

The aim of the work. To study the hormonal and immunological characteristics of the blood, the immunohistochemical features of the decidual membrane of the endometrium, to identify the mechanisms of the causes of miscarriage in early pregnancy. Study design: open independent prospective study. Material and methods. To achieve the stated goal, a retrospective analysis of 382 patient histories was conducted. A comparative prospective controlled study was performed in 258 women with habitual miscarriage. The first group included 198 women with habitual miscarriage. The control group consisted of 60 healthy fertile women with no history of miscarriage, with a full-term birth through the birth canal in the history, without deviation from the physiological course of pregnancy and the act of birth, as a result of which healthy children were born. At the second stage of the study, the main group was divided into two subgroups: Group 1 (n = 100): formed from patients who subsequently received a personalized, comprehensive examination and pre-pregnancy preparation, Group II (n = 98) - from patients who subsequently received a generally accepted volume of diagnostics and treatment. Results. When determining antibodies to human chorionic gonadotropin in the blood, an increase in antibodies was found in the first group, which was significantly different from the control group (p ≤ 0.001). The change in the above indicators indicates that the immune properties of the body during miscarriage are at a higher level. A change in the amount of TSH was noted in 36 (33.9%) women of the 1st group, it changed by 5.2-6.4. Only in the 1st group was the average TSH value relatively high compared to the control group (p≤0.001). One of the main indicators was the determination of the level of androgens, the average amount of testosterone in the 1st group was 4.85±0.087 nmol/l, in the 3rd group - 1.8. ±0.047 nmol/l. The indicators were significantly different compared to the 1st and 2nd groups (p≤0.001). The results of the immunohistochemical study of CD34 were assessed as weakly, moderately and highly positive. The minimum number of cells with a low expression index was detected in the very early stages. All 40 patients were tested for the presence of hCG receptors by immunohistochemistry, and a positive reaction was found in 100%. Positive staining was observed in 50% of mild and 50% of moderate expression. Progesterone is usually the only progestin. Immunohistochemistry revealed a positive reaction (100%), a highly positive reaction was observed in 10 patients, a moderately positive reaction in 50% of patients and a mildly positive reaction in 25%. Estrogen - out of 40 patients, 8 (20%) had a low positive reaction, 4 of them (10%) had a moderately positive reaction and 70% of 14 patients showed a negative reaction. Conclusion. Successful pregnancy requires the correct interaction between individual hormones and their corresponding receptors. Dysregulation or inadequate expression in one of these three compartments can lead to implantation failure or pregnancy loss.

Keywords: hCG antibodies, hormone receptors, immunohistochemistry, endometrium.

First page

623

Last page

632

For citation:Matrizayeva G.Dzh., Ikhtiyarova G.A. - MODERN APPROACH TO DIAGNOSIS OF MISTARRIAGE//New Day in Medicine 10(72)2024 623-632 https://newdayworldmedicine.com/en/new_day_medicine/10-72-2024

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