86 -11 (73) 2024 - Xikmatulalev R.Z. - DYNAMICS OF NEURON-SPECIFIC ENOLASE CONTENT IN THE BLOOD OF RATS AFTER SIMULATION OF SPINAL CORD INJURY

DYNAMICS OF NEURON-SPECIFIC ENOLASE CONTENT IN THE BLOOD OF RATS AFTER SIMULATION OF SPINAL CORD INJURY

Xikmatulalev R.Z. - Tashkent Medical Academy

Resume

We evaluated the protein levels of neuron-specific enolase (NSE) and S-100b in serum and cerebrospinal fluid (CSF) in an animal model of acute spinal cord injury and ascertained their relevance. Spinal cord injury was induced at the T8 level in rats. Enzyme-linked immunosorbent assay was used to measure the protein levels of NSE in both serum and CSF at different time points (30 min, 2 h, 6 h, 12 h and 24 h after induction of spinal cord injury). There existed a significant correlation between neurological deficits and the severity of spinal cord injury (p < 0.05). Compared with the control group, the protein levels of NSE and S-100b in serum and CSF significantly increased from 2 h after injury (p < 0.05) and reached a maximum at 6 h. Within a certain time window, the protein levels of NSE in serum and CSF were closely related to the severity of injury level (p < 0.05). The protein levels of NSE in serum and CSF significantly increased after experimental spinal cord injury in a time-dependent manner and thus may be considered specific biomarkers for acute spinal cord injury.

Key words: neuron-specific enolase; spinal cord injury; experiment; enzyme immunoassay

First page

497

Last page

501

For citation:Xikmatulalev R.Z. - DYNAMICS OF NEURON-SPECIFIC ENOLASE CONTENT IN THE BLOOD OF RATS AFTER SIMULATION OF SPINAL CORD INJURY//New Day in Medicine 11(73)2024 497-501 https://https://newdayworldmedicine.com/en/new_day_medicine/11-73-2024

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