174 -12 (74) 2024 - Kattakhodjaeva M.К., Ixtiyarova G.A. - METHODS FOR DIAGNOSING DISEASES OF THE UTERINE CERVIX

METHODS FOR DIAGNOSING DISEASES OF THE UTERINE CERVIX

Kattakhodjaeva M.К. - Tashkent State Dental Institute Uzbekistan

Ixtiyarova G.A. - Bukhara State Medical Institute named after Abu Ali ibn Sina

Karshiyeva E.E. - Tashkent State Dental Institute Uzbekistan

Navruzova N.O. - Bukhara State Medical Institute named after Abu Ali ibn Sina

Resume

Goal: was - the study of the state of the vaginal microbiocenosis and determination of the presence of diseases of the cervix, using the PCR method (Femoflor-16) - for the differential diagnosis of dysbiotic disorders and infectious and inflammatory diseases of genitalia in women. Methods: Clinical and laboratory examination of 105 patients with cervical diseases was carried out. All patients underwent the necessary diagnostic standard: examination of the cervix in the mirrors, cytological examination of smears from the ecto- and endocervix, polymerase chain reaction (PCR) for HPV, pH-metry, extended colposcopy, histological examination of cervical biopsies. Material for the study of vaginal microbiocenosis was collected from the posterolateral wall of the vagina, for HPV detection - from the cervical canal. The scraping was placed in an Eppendorf tube containing 1 ml of saline; storage and transportation of the material was carried out in accordance with the current regulatory documents. DNK was isolated using a PROBA-GS reagent kit (Standard Diagnostics, Bukhara). The study was carried out by PCR with detection of results in real time (RT-PCR) using Femoflor-16 reagents (Standard Diagnostics, Bukhara) in a detecting amplifier DT-96, according to the manufacturer's instructions, in the laboratory (Standard Diagnostics, g . Bukhara).

Keywords: colposcopy, base-line and pre-cancer diseases of cervix of the the uterus, diagnostics of diseases of cervix of the uterus, femoflor-16.

First page

906

Last page

914

For citation:Kattakhodjaeva M.К., Ixtiyarova G.A., Karshiyeva E.E., Navruzova N.O. - METHODS FOR DIAGNOSING DISEASES OF THE UTERINE CERVIX//New Day in Medicine 12(74)2024 906-914 https://https://newdayworldmedicine.com/en/new_day_medicine/12-74-2024

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