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  • 55 -1 (75) 2025 - Musashaykhov Х.Т., Nazarov I.R. - EVALUATION OF THE VALUE OF THE C786T POLYMORPHISM OF THE NOS3 GENE IN THE RISK OF PURULENT-NECROTIC LESIONS OF THE LOWER EXTREMITIES IN PATIENTS WITH DIABETES MELLITUS

55 -1 (75) 2025 - Musashaykhov Х.Т., Nazarov I.R. - EVALUATION OF THE VALUE OF THE C786T POLYMORPHISM OF THE NOS3 GENE IN THE RISK OF PURULENT-NECROTIC LESIONS OF THE LOWER EXTREMITIES IN PATIENTS WITH DIABETES MELLITUS

EVALUATION OF THE VALUE OF THE C786T POLYMORPHISM OF THE NOS3 GENE IN THE RISK OF PURULENT-NECROTIC LESIONS OF THE LOWER EXTREMITIES IN PATIENTS WITH DIABETES MELLITUS

Musashaykhov Х.Т. - Andijan State Medical Institute

Nazarov I.R. - Andijan State Medical Institute

Musashaykhova Sh.M. - Andijan State Medical Institute

Begmatov A.X. - Andijan branch of the Republican Specialized Scientific and Practical Medical Center of Endocrinology

Resume

A total of 201 people were involved in the implementation of the goals and objectives of our scientific research. The prognosis of the development of purulent-necrotic diseases of the lower extremities in patients with diabetes mellitus was analyzed by studying the frequency of the C-786T polymorphism of the NOS3 gene in 103 patients who made up the main group. The comparison group consisted of 98 conditionally healthy individuals. Genomic DNA preparations were used as material for the control samples, corresponding to the sex and age of the patients in the study group, who do not have the clinical signs of the above-mentioned pathologies in themselves and their close relatives. These samples were independently isolated from "conditionally healthy" individuals and are stored in the DNA bank of the Republican Specialized Scientific and Practical Medical Center of Hematology of the Ministry of Health of the Republic of Uzbekistan. The C-786T polymorphism of the NOS3 gene was investigated using a Rotor-Gene Q (Quagen, Germany) instrument using a commercial reagent kit from "Litech" LLC

Key words: NOS3 gene C-786T polymorphism, diabetes mellitus, lower limbs, DNA, genotype, allele.

First page

335

Last page

339

For citation:Musashaykhov Х.Т., Nazarov I.R., Musashaykhova Sh.M., Begmatov A.X. - EVALUATION OF THE VALUE OF THE C786T POLYMORPHISM OF THE NOS3 GENE IN THE RISK OF PURULENT-NECROTIC LESIONS OF THE LOWER EXTREMITIES IN PATIENTS WITH DIABETES MELLITUS//New Day in Medicine 1(75)2025 335-339 https://https://newdayworldmedicine.com/en/new_day_medicine/1-75-2025

List of References

  1. American Diabetes Association. Standards of medical care in diabetes – 2019 //Diabetes Care 2019;42(1):1-193.
  2. Amin N., Doupis J. Diabetic foot disease: From the evaluation of the "foot at risk" to the novel diabetic ulcer treatment modalities //Wld J. Diab. 2020;7(7):153-164.
  3. Baba M., Davis W.A., Norman P.E., Davis T.M. Temporal changes in the prevalence and associates of foot ulceration in type 2 diabetes: the Fremantle Diabetes Study //J. Diabetes Complications. 2015;29(3):356-61.
  4. Babadjanov B.D., Matmurotov К.J., Atajanov T.Sh. Efficacy of Minimally Invasive Procedures in the Treatment of Lower Extremities Diabetic Gangrene //Amer. J. Med. Sci. 2019;9(5):163-166.
  5. Balmer H., Mahler F., Do D.D. et.al. Ballon angioplasty in chronic critical limb ischemia: factors affecting clinical and angiographic outcome //J. Endovasc. Ther. 2022:9(4):403-410.
  6. Mohammed S.I., Mikhael E.M., Ahmed F.T. et al. Risk factors for occurrence and recurrence of diabetic foot ulcers among Iraqi diabetic patients //Diab. Foot Ankle. 2021; 7 p.
  7. Remington A.C., Hernandez-Boussard T., Warstadt N.M. et al. Analyzing treatment aggressiveness and identifying high-risk patients in diabetic foot ulcer return to care //Wound Repair Regen. 2016;24(4):731-6.
  8. Xu Z., Ran X. Diabetic foot care in China: challenges and strategy //Lancet Diabetes Endocrinol. 2021;1(5):397-398.
  9. Yen C.J., Beamer BA, Negri C, Silver K, Brown KA, Yarnall DP, et al. Molecular scanning of the human Peroxisome proliferator activated receptor g (hPPARg) gene in diabetic Caucasians: identification of a Pro12Ala PPARg2 missense mutation //Biochem Biophys Res Commun. 1997;241(2):270-274.
  10. Zhan L.X., Branco B.C., Armstrong D.G., Mills J.L. The Society for Vascular Surgery lower extremity threatened limb classification system based on Wound, Ischemia, and foot Infection (WIfI) correlates with risk of major amputation and time to wound healing //J. Vasc. Surg. – 2015;61(4):939-44.

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