93 -7 (81) 2025 - Mukhitdinova K.O., Aleуnik V.A. - INFLUENCE OF UNFRACTIONATED AND LOW-MOLECULAR WEIGHT HEPARIN ON LEUCOCYTE DNA METHYLATION IN WOMEN WITH GENITAL INFECTIONS AND MISCARRIAGE

INFLUENCE OF UNFRACTIONATED AND LOW-MOLECULAR WEIGHT HEPARIN ON LEUCOCYTE DNA METHYLATION IN WOMEN WITH GENITAL INFECTIONS AND MISCARRIAGE

Mukhitdinova K.O. - Andijan State Medical Institute, Andijan branch of the Institute of Human Immunology and Genomics of the Academy of Sciences of the Republic of Uzbekistan

Aleуnik V.A., - Andijan State Medical Institute, Andijan branch of the Institute of Human Immunology and Genomics of the Academy of Sciences of the Republic of Uzbekistan

Babich S.M. - Andijan State Medical Institute, Andijan branch of the Institute of Human Immunology and Genomics of the Academy of Sciences of the Republic of Uzbekistan

Negmatshaeva H.N. - Andijan State Medical Institute, Andijan branch of the Institute of Human Immunology and Genomics of the Academy of Sciences of the Republic of Uzbekistan

Zhuraev B.M. - Andijan State Medical Institute, Andijan branch of the Institute of Human Immunology and Genomics of the Academy of Sciences of the Republic of Uzbekistan

Resume

The work studied the effect of unfractionated and low-molecular-weight heparin on leukocyte DNA methylation in women with genital infections and miscarriage. It was concluded that women with genital infections and early miscarriages (comparison group) without pregravid therapy had a pronounced pathological phenotype. It was characterized relative to the control (women with infections and full-term pregnancy) by hyperactivation of proteolysis manifested by a reliable increase in MMP and a decrease in IMMP, progressing during pregnancy, in addition, by the hypermethylating epigenetic status in leukocytes, expressed in a significant increase in DNMT1 activity and the level of global DNA methylation (5-methyl-2'-deoxycytidine) relative to the control, increasing during gestation. Imbalance of the immune response represented by a sharp dominance of proinflammatory cytokines in the blood (TNF-α, IL-1β) and suppression of anti-inflammatory IL-10 relative to the control at all stages. With pregravid use of LMWH (Clexane), a moderate numerical decrease in the DNMT1 level was noted in leukocytes at week 12 relative to the comparison group without statistical significance, at a slightly low initial level. Numerical decrease in the level of 5-methyl-2'-deoxycytidine at week 12. The initial level and increase during pregnancy were also numerically lower than in the comparison group. In the blood, the dynamics of IL-10 was more stable without a significant decrease. The level of TNF-α was higher than in the group with UFH, but lower than in the comparison group. With pregravid use of UFH, a significant numerical decrease in the level of DNMT1 in leukocytes was noted at week 12 relative to the comparison group, where statistical significance was not achieved, but the value was significant. The initial level was also significantly lower. A pronounced numerical decrease in the level of 5-methyl-2'-deoxycytidine at week 12 (the value is significant). The initial level was significantly lower, and the increase during pregnancy was less than in the comparison group. In the blood, IL-10 decreased significantly, the level of TNF-α was lower than in the comparison group and lower than with LMWH.

Keywords: leukocyte DNA methylation, genital infections, interleukins, early pregnancy, miscarriage, heparin.

First page

494

Last page

502

For citation:Mukhitdinova K.O., Aleуnik V.A., Babich S.M., Negmatshaeva H.N., Zhuraev B.M. - INFLUENCE OF UNFRACTIONATED AND LOW-MOLECULAR WEIGHT HEPARIN ON LEUCOCYTE DNA METHYLATION IN WOMEN WITH GENITAL INFECTIONS AND MISCARRIAGE//New Day in Medicine 7(81)2025 494-502 https://newdayworldmedicine.com/en/new_day_medicine/7-81-2025

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