42 -8 (82) 2025 - Rasulova N.R., Temirova N.R. - STUDY OF IMMUNOHISTOCHEMICAL FEATURES OF THYMUS TISSUE AFTER EXPERIMENTAL CHEMOTHERAPY

STUDY OF IMMUNOHISTOCHEMICAL FEATURES OF THYMUS TISSUE AFTER EXPERIMENTAL CHEMOTHERAPY

Rasulova N.R. - Bukhara State Medical Institute named after Abu Ali ibn Sina

Temirova N.R. - Bukhara State Medical Institute named after Abu Ali ibn Sina

Resume

This study examined the immunohistochemical changes that develop in the thymus tissue of white outbred rats following experimental chemotherapy. The aim of the research was to identify alterations in the morphostructural and immunophenotypic characteristics of the thymus under the influence of a cytostatic drug and to assess their immunological consequences. In the experiment, paclitaxel was used as the chemotherapeutic agent at a specified dose. Immunohistochemical analysis of thymus tissues was performed using CD4 and CD138 markers. Morphometric assessment included determining the ratio of cortical to medullary zones, the density of lymphoid cells, and the morphological state of stromal components, followed by evaluation of the degree of their changes. These findings highlight the morphological basis of immune system weakening and provide a scientific foundation for developing post-chemotherapy immunorehabilitation strategies.

Keywords: thymus, chemotherapy, immunohistochemistry, CD4, CD138, lymphoid cells, macrophage activity

First page

236

Last page

243

For citation:Rasulova N.R., Temirova N.R. - STUDY OF IMMUNOHISTOCHEMICAL FEATURES OF THYMUS TISSUE AFTER EXPERIMENTAL CHEMOTHERAPY//New Day in Medicine 8(82)2025 236-243 https://newdayworldmedicine.com/en/new_day_medicine/8-82-2025

List of References

1. Abbas A.K., Lichtman A.H., Pillai S. Cellular and Molecular Immunology. 10th ed. – Philadelphia: Elsevier, 2021. – 608 p.
2. Anderson G., Jenkinson E.J. Lymphostromal interactions in thymic development and function // Nature Reviews Immunology. – 2001. – Vol. 1, No. 1. – P. 31–40.
3. Boyd R.L., Tucek C.L., Godfrey D.I., et al. The thymic microenvironment // Immunology Today. – 1993. – Vol. 14, No. 9. – P. 445–459.
4. Heng T.S.P., Chidgey A.P., Boyd R.L. Getting back at nature: understanding thymic development and function through the mouse // Immunological Reviews. – 2010. – Vol. 236, No. 1. – P. 170–190.
5. Miller J.F.A.P. The discovery of thymus function and of thymus-derived lymphocytes // Immunological Reviews. – 2002. – Vol. 185. – P. 7–14.
6. Пальцев М.А., Северинов Д.А., Зыков А.В. Иммунная система и стресс: роль тимуса в регуляции иммунитета // Патологическая физиология и экспериментальная терапия. – 2018. – № 62 (4). – С. 101–109.
7. Rasooly L., Herberman R.B., Whiteside T.L. Immunomodulatory effects of paclitaxel on murine immune cells // Cancer Immunology, Immunotherapy. – 1998. – Vol. 46, No. 4. – P. 213–220.
8. Tanaka H., Matsumoto Y., Udagawa T., et al. Thymic involution and immune reconstitution after chemotherapy in animal models // Experimental Hematology. – 2019. – Vol. 70. – P. 25–34.
9. Williams K.M., Hakim F.T., Gress R.E. T cell immune reconstitution following lymphodepletion // Seminars in Immunology. – 2007. – Vol. 19, No. 5. – P. 318–330.
10. Zhang L., Su X., Pan Z., et al. Chemotherapy-induced thymic involution and immune suppression: molecular mechanisms and clinical implications // Frontiers in Immunology. – 2021. – Vol. 12. – Article 652.

    file

    download