148 -10 (84) 2025 - Ulugbekova G.J., Mamajonov Z.A. - EVALUATION OF THE POLYPHENOL COMPOSITION AND ANTITUMOR ACTIVITY OF MELISSA OFFICINALIS AND SALVIA SPLENDENS USING THE HPLC METHOD

EVALUATION OF THE POLYPHENOL COMPOSITION AND ANTITUMOR ACTIVITY OF MELISSA OFFICINALIS AND SALVIA SPLENDENS USING THE HPLC METHOD

Ulugbekova G.J. - Andijan State Medical Institute

Mamajonov Z.A. - Andijan State Medical Institute

Mamatova I.Y. - Andijan State Medical Institute

Resume

In this study, the polyphenol composition and anticancer activity of ethanol extracts obtained from Melissa officinalis (lemon balm) and Salvia splendens (scarlet sage) were investigated using high-performance liquid chromatography (HPLC). The results showed that Melissa officinalis contained high levels of salicylic acid (273.65 mg/100 g), rutin (6.21 mg/100 g), and gallic acid (0.67 mg/100 g), while rosmarinic acid (18.45 mg/100 g), chlorogenic acid (7.82 mg/100 g), and quercetin (0.93 mg/100 g) predominated in the Salvia splendens extract. Both extracts demonstrated strong antioxidant activity (DPPH 82.4 ± 3.7%), reduced cancer cell proliferation, and enhanced apoptosis. It was determined that the polyphenols in these plants modulate NF-κB, MAPK, and p53 signaling pathways, reduce oxidative stress, and balance immune responses. Therefore, extracts of Melissa officinalis and Salvia splendens represent promising natural antioxidant and phytotherapeutic agents for cancer prevention and adjunct therapy.

Keywords: Melissa officinalis; Salvia splendens; polyphenols; phenolic compounds; HPLC; antioxidant activity; apoptosis; antitumor effect; NF-κB; MAPK; p53.

First page

839

Last page

843

For citation: Ulugbekova G.J., Mamajonov Z.A., Mamatova I.Y. - EVALUATION OF THE POLYPHENOL COMPOSITION AND ANTITUMOR ACTIVITY OF MELISSA OFFICINALIS AND SALVIA SPLENDENS USING THE HPLC METHOD//New Day in Medicine 10(84)2025 839-843 https://newdayworldmedicine.com/en/new_day_medicine/10-84-2025

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