165 -2 (88) 2026 - Аkhmedova L.M., Ibadullaeva N.S. - DEVELOPMENT AND OPTIMIZATION OF A METHOD FOR RAPID EXTRACTION OF HPV DNA

COMPREHENSIVE PREVENTION OF DENTAL DISEASES IN MECHANICAL ENGINEERING WORKERS

Аkhmedova L.M. - The Research Institute of Virology of the Republican specialized scientific and practical medical center of epidemiology, microbiology, infectious and parasitic diseases

Ibadullaeva N.S. - The Research Institute of Virology of the Republican specialized scientific and practical medical center of epidemiology, microbiology, infectious and parasitic diseases

Аkhmedova Sh.X. - The Research Institute of Virology of the Republican specialized scientific and practical medical center of epidemiology, microbiology, infectious and parasitic diseases

Тurabova N.R. - Human papillomavirus is the leading etiologic factor in cervical cancer, necessitating the development of rapid and reliable molecular methods for its laboratory detection. The highest diagnostic efficiency for HPV DNA detection was achieved using a rapid protocol based on 0,05% SDS and 0,02% Tris-HCl with the addition of nuclease-free water after the incubation step (sensitivity - 91%, specificity - 96%). Further studies are required to further improve diagnostic sensitivity and specificity.

Keywords: HPV, DNA, real-time PCR, SDS, sensitivity, specificity.

First page

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Last page

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For citation:Аkhmedova L.M., Ibadullaeva N.S., Аkhmedova Sh.X., Тurabova N.R. - COMPREHENSIVE PREVENTION OF DENTAL DISEASES IN MECHANICAL ENGINEERING WORKERS//New Day in Medicine 2(88)2026 888-893 https://newdayworldmedicine.com/en/new_day_medicine/2-88-2026

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