13 -4 (90) 2026 - Rakhmanova N.Q., Aripov O.A. - THE IMPORTANCE OF PCR, CYTOLOGICAL, AND MICROBIOLOGICAL EXAMINATIONS IN THE EARLY DIAGNOSIS OF GENITAL PAPILLOMAVIRUS INFECTION IN WOMEN OF REPRODUCTIVE AGE

THE IMPORTANCE OF PCR, CYTOLOGICAL, AND MICROBIOLOGICAL EXAMINATIONS IN THE EARLY DIAGNOSIS OF GENITAL PAPILLOMAVIRUS INFECTION IN WOMEN OF REPRODUCTIVE AGE

Rakhmanova N.Q. - Tashkent State Medical University

Aripov O.A. - Center for Professional Development of Medical Personnel Uzbekistan

Resume

Human papillomavirus, especially its high-oncogenic-risk types, may lead to the development of pathological changes in the cervix and other genital organs. Therefore, early diagnosis of this infection, assessment of risk factors, and prevention of complications remain urgent issues in modern medicine. This article analyzes current approaches to the early diagnosis of genital papillomavirus infection in women of reproductive age, including cytological, molecular-genetic, and microbiological diagnostic methods. The importance of PCR, cytological, and microbiological examinations in the early diagnosis of genital papillomavirus infection in women of reproductive age. The study considered HPV16 PCR results, microbiological reports on vaginal microflora, and cytological materials. The findings support the value of a combined laboratory approach.

Keywords: HPV, HPV16, PCR, cytology, Pap test, vaginal microflora, microbiological examination.

First page

83

Last page

87

For citation:Rakhmanova N.Q., Aripov O.A. - THE IMPORTANCE OF PCR, CYTOLOGICAL, AND MICROBIOLOGICAL EXAMINATIONS IN THE EARLY DIAGNOSIS OF GENITAL PAPILLOMAVIRUS INFECTION IN WOMEN OF REPRODUCTIVE AGE//New Day in Medicine 4(90)2026 83-87 https://newdayworldmedicine.com/en/new_day_medicine/4-90-2026

List of References

1. World Health Organization. Cervical cancer. Geneva: WHO; 2025.
2. World Health Organization. WHO guideline for screening and treatment of cervical pre-cancer lesions for cervical cancer prevention: use of dual-stain cytology to triage women after a positive test for human papillomavirus (HPV). Geneva: WHO; 2024.
3. World Health Organization. Self-care interventions for health and well-being: HPV self-sampling as part of cervical cancer screening. Geneva: WHO; 2023.
4. Wei F, Georges D, Man I, Baussano I, Clifford GM, et al. Causal attribution of human papillomavirus genotypes to invasive cervical cancer worldwide: a systematic analysis of the global literature. Lancet. 2024.
5. International Agency for Research on Cancer. Global attribution of HPV genotypes to invasive cervical cancer: a systematic analysis. Lyon: IARC; 2024.
6. National Cancer Institute. HPV and Cancer. Bethesda: NCI; 2025.
7. Ouh YT, Min KJ, Choi CH, Lee JW, Kim BG, et al. Enhancing cervical cancer screening: review of p16/Ki-67 dual staining as a promising triage strategy. Cancers (Basel). 2024;16(2). doi:10.3390/cancers16020406.
8. Clarke MA, Cheung LC, Castle PE, et al. Recommendations for use of p16/Ki67 dual stain for management of individuals testing positive for human papillomavirus. J Low Genit Tract Dis. 2024;28(1):e1–e8. doi:10.1097/LGT.0000000000000769.
9. Harper DM, et al. p16/Ki-67 dual stain triage of individuals positive for HPV to detect cervical precancer: review and clinical perspective. Ther Adv Vaccines Immunother. 2025.
10. Schreiberhuber L, Barrett JE, Wang J, et al. Cervical cancer screening using DNA methylation triage in a real-world population. Nat Med. 2024;30(8):2251–2257. doi:10.1038/s41591-024-03058-5.

    file

    download