47 -3 (41) 2022- Khasanova D.A. — INTRAORGAN CHANGES OF RAT SPLEEN VESSELS IN EARLY POSTNATAL ONTOGENESIS

INTRAORGAN CHANGES OF RAT SPLEEN VESSELS IN EARLY POSTNATAL ONTOGENESIS

Khasanova D.A. Bukhara State Medical Institute named after Abu Ali Ibn Sino

Resume

The experiment was carried out on 90 male mongrel rats in the newborn and at 6, 11, 16 and 21 days of age. To study the histotopography of the processes, polyclonal antibodies to CD3 and CD20 (Ventana, Switzerland) were used at a dilution of 1:100, carried out on histological glasses with an adhesive coating (Ventana, Switzerland). In the preparations, the spleen structures were examined morphometrically using an ocular micrometer. The diameter of the periarterial lymphatic couplings of the spleen, lymph nodes and their germinal centers, the width of the mantle, marginal and periarterial zones of lymph nodes, the relative area of connective tissue elements and white pulp (relative to the total area of the cut) were measured.

The spleen of newborn baby rats is functionally immature, lymph nodes are not fully formed, it is difficult to distinguish all zones in them and germinative centers are not determined.  When performing IHC with CD 20 markers, it was found that the number of LF without breeding centers in the spleen of the control group of rats reaches a maximum by 6 days of age, and with breeding centers by 11 days of age.

Key words: immune system, spleen, lymphoid follicles, early postnatal ontogenesis.

First page

228

Last page

232

For citation: Khasanova D.A. INTRAORGAN CHANGES OF RAT SPLEEN VESSELS IN EARLY POSTNATAL ONTOGENESIS //New Day in Medicine 3(41)2022 228-231 https://clck.ru/frX6t

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