12 -5 (43) 2022 — Tursumetov A.A., Isakov Sh.Sh. — ANTIMICROBIAL EFFECT OF DIODE LASER RADIATION ON MICROFLORA SENSITIZED WITH METHYLENE BLUE IN PURULENT ENDOBRONCHITIS in vitro

ANTIMICROBIAL EFFECT OF DIODE LASER RADIATION ON MICROFLORA SENSITIZED WITH METHYLENE BLUE IN PURULENT ENDOBRONCHITIS in vitro

Tursumetov A.A., Tashkent Pediatric Medical Institute

Isakov Sh.Sh. Tashkent Pediatric Medical Institute

Resume

The article presents the results of studying the spectrum of microbes in purulent endobronchitis, which developed during prolonged mechanical ventilation after emergency operations on the abdominal organs. It was established that the main pathogens of endobronchitis were: Escherichia coli – 18.2%, Staphylococcus aureus – 14.2%, Acinetobacter spp. – 6.2%, Klebsiella pneumonia – 6%, Candida spp. – 10.7%, Pseudomonas aeruginosa – 7.4%. Staphylococcus aureus MR – 3.3%. It was shown that the photodynamic effect on the microflora using the MS photosensitizer at a concentration of 0.05%, at a laser radiation energy density of 25-35 J/cm2, exposure time of 99-180 sec, has a bactericidal effect against gram+ and gram- bacteria, including number for MRSA. The authors believe that it is expedient to further research in the experiment under in vivo conditions to study the effectiveness of PDT treatment for the sanitation of the bronchial tree in purulent endobronchitis.

Keywords: antimicrobial action, diode laser radiation, microflora, methylene blue, purulent endobronchitis

First page

64

Last page

73

For citation: Tursumetov A.A., Isakov Sh.Sh. ANTIMICROBIAL EFFECT OF DIODE LASER RADIATION ON MICROFLORA SENSITIZED WITH METHYLENE BLUE IN PURULENT ENDOBRONCHITIS in vitro //New Day in Medicine 5(43)2022 64-73 https://clck.ru/haVYX

LIST OF REFERENCES:

1. Васильєв Н.Е.Антимикробная фотодинамическая терапия / Н.Е. Васильєв, А.П. Огиренко // Лазерная медицина. — 2002.— Т. 6, № 4.— С. 32–38.
2. Жданов О.С., Красноженов Е.П., Соснин Э.А. с соавт. Антибиотикорезистентность штаммов Pseudomonas aeruginosa с разной способностью к синтезу пиоционина. //Альманах клинической медицины 2013. -№28.-С.13-17.
3. Ибадов Р.А., Шаниева З.А., Арифжанов А.Ш., Эшонходжаев О.Д.  с соавт.
4. Оригинальные биотехнологии в профилактике и лечении бронхолегочных осложнений у больных находящихся на пролонгированной ИВЛ. Journal of Microbiology Research 2014, 4(1): 14-17 DOI: 10.5923/j.microbiology.20140401.03.
5. Мудрак Д.А. Молекулярно-генетические особенности устойчивости к бета-лактамным антибиотикам грамотрицательных микроорганизмов – возбудителей нозокомиальных инфекций. /Автореф. дисс. канд. биол. наук. М., 2010
6. Проценко Д.Н., Белоцерковский Б.З. Нозокомиальная пневмония: состояние проблемы //Инфекции в хирургии. Том 7, Приложение №1, 2009; 6-10.
7. Странадко Е.Ф. Основные этапы развития фотодинамической терапии в России //Фотодинамическая терапия и фотодиагностика.- 2015.-№1.- С. 3-10.
8. Тихов Г.В. Фотодинамическая терапия в лечении перитонита: автореф. дис. … канд. мед. наук: 14.01.17/ Тихов Григорий Вячеславович. – М., 2014. – 24с.
9. Bradford PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev. 2001 Oct;14(4):933-51, PMID:11585791
10. Dougherty T.J., Thoma R. E., Boyle D. G. et al. Photoradiation therapy of malignant tumors; role of the laser // Pratesi R., Sacchi C.A. (eds.) Lasers in photomedicine and photobiology. – New York: Springer-Verlag, 1980. – P. 67-75.
11. Polycationic photosensitizer conjugates: effects of chain length and Gram classification on the photodynamic inactivation of bacteria / M. Hamblin, D. O’Donnell, N. Marthy [et al.] // J. of Antimicrobial Chemotherapy.  2002. Vol. 13, № 49. Р. 941–951.
12. Hajim K.I., Salih D.S.,Rassam Y.Z. Laser light combined with a photosensitizer may eliminate mathicilline-resistant strains of Staphilococcus aureus|| Lasers Med/Sci.-2010.-Vol.25 (5).-p.743-748.