80 -1 (29/2) 2020 — Narzulloeva N.S., Olimov J.N., — PATHOGENETIC SUBSTANTIATION OF DIFFERENTIATED IMMUNOMODULATION IN WOMEN WITH UTERINE MYOMA AND INFERTILITY

PATHOGENETIC SUBSTANTIATION OF DIFFERENTIATED IMMUNOMODULATION IN WOMEN WITH UTERINE MYOMA AND INFERTILITY

Narzulloeva N.S., Bukhara state medical Institute.

Olimov J.N., Bukhara state medical Institute.

Resume,

Objective.. To assess the role of immunological factors in the formation of clinical variants of uterine fibroids to develop reasonable methods of differentiated corrective therapy

Subject and methods. In the period 2016-2019 years in the Bukhara region were analyzed 475 questionnaires of women with infectious-inflammatory diseases of the pelvic organs. 250 of them (52, 6%) were identified MM different shape and location. For this purpose, we studied 115 women of reproductive age with inflammatory diseases of the pelvic organs. To configure ELISA was used Immunological diagnostic: cytokines in the serum by ELISA (IL-2, IL- 6, IL-18, MCP-1   and IL-4) Lactoferrin method your oven CD25, CD71, CD95     with monoclonal antibodies LT series. Clinical and anamnestic, instrumental (ultrasound c doplerometriya, MRI) Laboratory – oak, CL, TR hormones – FSH, LH, prolactin, progesterone, estradiol, T3, T4, TSH, cortisol

Results. For women with uterine fibroids is characterized by increased levels of FSH and LH decrease, except for women with infertility who have reduced levels of FSH and LH increased with a sharp increase in levels of prolactin and progesterone. Estradiol significantly elevated in all the surveyed women, except for women with infertility. For all women with uterine fibroids characteristic imbalance of thyroid hormones (T3, T4 and TSH). In women with large uterine fibroids on the background of menorrhagia and pain, cortisol levels significantly increased. In women with infertility on the background of symptomatic uterine fibroids revealed metabolic syndrome with elevated cholesterol and triglycerides. Only women with asymptomatic uterine fibroids consistently small size there was a significant increase in the level of activated CD71+lymphocytes and reduced the readiness of lymphocytes to apoptosis (CD95+), (P<0.001), while the level of lactoferrin was significantly reduced.

Conclusion. The level of CD71+cells was the lowest at an elevated level of lactoferrin in women with menorrhagia (P<0.001). Infertility level of lymphocyte early activation (CD25+) was significantly high. Maximum IL-6 level was observed in women with infertility and at larger sizes of fibroids in women with asymptomatic uterine fibroids on the background of significantly increased level of progesterone. The level of TNF was elevated in all forms of uterine fibroids, but is highest at larger sizes of fibroids in women with menorrhagia there is a direct relationship of increased level of cortisol with an increase in proinflammatory cytokine IL-18 and reduction of hemoglobin. For large size uterine fibroids and menorrhagia, the level of IL-8 increased sharply on the background of high levels of estradiol.

Key words: cytokine, activation marker, enzyme-linked immunosorbent assay, female, uterine fibroids

First page

290

Last page

294

For citation: Narzulloeva N.S., OlimovJ.N. Pathogenetic substantiation of differentiated immunomodulation in women with uterine myoma and infertility//New Day in Medicine 1(29)2020 290-294 https://cutt.ly/IvNELZ3

List of References

1. Егунян А.Ш., Клинико-морфологические особенности мио­мы матки, требующей хирургического лечения, в возраст­ном аспекте: автереф. дис. канд. мед. наук. Челябинск 2008; 143.
2. Giraudet G., Lucot J.P. et al. Exsept fertility, place of myomectomy in perimenopause and after menopause //Gynecol, Obstet Biol Reprod. 2011; 40(8): 902-917.
3. Беженарь В.Ф., Цыпурдеева А.А., Долинский А.К., Бочориш- вилли Р.Г., Лапароскопическая гистерэктомия – семилетний опыт. //Ж. Акушерства и женских болезней. 2011; 4: 12-19.
4. Нарзуллаева Н.С. Мусаходжаева Д.А., Ихтиярова Г.А., Gynecological and somatic history of women with uterine myoma according to retrospective analysis //European journal of pharmaceutical and medical research //SJIF Impact Factor 4.897.C.153-157.
5. Segars J.H. et al Proceedings from the Third National Institutes of Health International Congress on Advances in Uterine Leiomyoma Research: comprehensive review, conference summary and., 2014.
6. Хмельницкий О.К. Цитологическая и гистологическая диагности­ка заболеваний шейка тело матки. -СП.: СОТИС,1999; 272-274.
7. Somigliana E. et al. Fibroids and female reproduction: a critical analysis of the evidence //Human 2007 Reproduction -2007; 13(5): 465-76.
8. Brady D.D, Dunson D.B., Hill M.C., Cousins D., Schectman J.M. High cumulative incidence of uterine leiomyoma in black and white woman :ultrasound evidence //Am. J.Obstet. Gynecol. 2003; 188(1): 100-7.
9. Нарзуллоева Н.С. Пролиферация и апоптоз миофибробластов в патогенезе лейомиомы /Сотникова Н.Ю., Воронин Д.Н., Анциферова Ю.С., Малышкина А.И., Нагорный С.Н., Нар­зуллоева Н.С. //Назарий ва клиник тиббиёт 2019; 1. Тошкент.
10. Ciavattina A. et al. Uterine Fibroids: Pathogenesis and Interactions with Endometrium and endomiometrial Junction //Obstet Gynecol Int. 2013.
11. Коган И.Ю., Беженарь В.Ф., Долинский А.К., Чмаро М.Г., Эффективность вспомогательных методов репродукции у больных с миомой матки //Акушерства и женских болез­ней – 2012; 113-118.
12. Нарзуллаева Н.С., Абдурахманов М.М., Магзумова Н.М. Па­раметры гуморольного звено иммунной системы у пациен­ток с миомой матки // Журнал теоретической и клиничес­кой медицины. Ташкент, 2018; 4(3): 91-93.